Strain-Specific Identification ofBifidobacterium bifidumOLB6378 by PCR

Detection and strain identification of Actinobacillus actinomycetemcomitans by nested PCR.

By using PCR, Actinobacillus actinomycetemcomitans strains were identified directly from plaque samples without the need to isolate or culture bacteria. DNA fragments were generated by a nested, two-step PCR amplification of the ribosomal spacer region between the 16S and 23S rRNA genes. For the first amplification, primers homologous to sequences common to all bacterial species were used. This...

Serotype Identification of Recent Iranian Isolates of Infectious Bronchitis Virus by Type-Specific Multiplex RT-PCR

Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers.

In the present work, strain-specific PCR primers for Lactobacillus rhamnosus Lc 1/3 are described. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. They were screened for specificity by hybridization with DNA from 11 L. rhamnosus strains. A 613-bp RAPD marker found to be strain-specific was sequenced, and a primer pair specific to L....

CLN3 by allele specific PCR

The recent isolation of the CLN3 gene involved in Batten disease (juvenile neuronal ceroid lipofuscinosis) creates possibilities for direct detection of mutations which can confirm or indicate the clinical diagnosis of Batten disease. We have designed a rapid and reliable allele specific PCR test for the detection of the major deletion, which can be used in carrier diagnosis, presymptomatic dia...

identification of toxoplasma gondii strain in human and mouse in urmia by pcr-rflp

infection by toxoplasma gondii is widespread inhumans and many other species of warm-blooded animals. it can cause significant morbidity and mortality in the developing fetus and in immunocompromised individuals,including humans with acquired immunodeficiency syndrome (aids) or submitted to cancer chemotherapy.among livestock, sheep and goat are more widely infected with t. gondii. this parasit...